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Rapamycin treatment of infected cells alone does not affect a wild-type Cryptosporidium infection in vitro (Fig S6A), however the addition of rapamycin to an infection with this transgenic strain abolished the development of male and female parasites, and skewed asexual parasites towards smaller, earlier stages of development (Fig 4H-I)

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After two rounds of screening a total of 7970 Keio strains, we confirmed nine bacterial mutants that enhanced CPBL-1 expression relative to the parental strain (Fig