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Small molecule NNMT inhibitors have been developed and tested in animals, including tricyclic compounds aimed at metabolic disease (the Scientific Reports paper describing novel tricyclic small molecule inhibitors of nicotinamide N-methyltransferase for metabolic disease), and NNMT inhibition has been combined with calorie restriction in diet induced obese mice (the study of a reduced calorie diet combined with NNMT inhibition establishing a distinct microbiome in diet induced obese mice)

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[3][16][17] In conditions such as obesity, type 2 diabetes, and aging, NNMT is overexpressed in adipose tissue and skeletal muscle, where

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