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JAMA , 331(14), 1230–1231

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2.5 Immunostaining of RBC-NOS serine 1177 residue The immunostaining protocol has been published in detail elsewhere ( 2.6 S-nitrosylation of RBC cytoskeletal α- and β-spectin The S-Nitrosylated Protein Detection Assay (Cayman Chemical, Ann Arbor, United States) was performed following established protocols ( 2.7 Parameters of oxidative stress: free ROS/RNS levels and total antioxidant capacity RBC free radical content was measured in 4 × 10 6 RBC using the OxiSelect In Vitro ROS/RNSAssay Kit (Cell Biolabs Inc., San Diego, United States), as previously described ( Total antioxidant capacity (TAC) was measured in 1 × 10 7 RBC using a respective Assay Kit (Abcam, Cambridge, United Kingdom)