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These methods see more use in protein p K a prediction, where direct QM-based workflows are generally impractical
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This concept has been utilized to monitor covalent adduct formation of covalent inhibitors NSC697923 and BAY 11-7082 with E2 ubiquitin conjugating enzyme Ubc13 [143]: elimination of 4-methylbenzene-sulfinic acid upon covalent thiol addition can be monitored by an increase in absorbance in the UV-visible spectrum directly related to covalent adduct formation
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