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Transepithelial resistance measurements Caco-2 cells were seeded onto 12 mm polycarbonate membrane inserts (PCF membrane inserts, Millipore, Germany) and bathed in DMEM/F12 medium which contained phenol red, 10% FBS, penicillin (100 units/ml) and streptomycin (10 µg/ml)

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parallel rings pre-incubated with the nitric oxide synthase inhibitor L-NAME at 10^-6 M Reported finding Concentration-dependent reduction of phenylephrine-induced contraction was recorded in both groups and was significantly greater in endothelium-intact rings