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100 μg/mL) with or without Escherichia coli lipopolysaccharide (LPS) (100 ng/mL), the control group was treated with equal amount of phosphate buffered saline (PBS) for 24 h at 37°C

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Inhibition of cholesterol esterification in T cells by genetic ablation or pharmacological inhibition of ACAT1 (a key cholesterol esterase) can lead to potentiated effector function and enhanced proliferation of CD8(+) T cells by increasing plasma membrane cholesterol levels, which causes enhanced T-cell receptor clustering and signaling as well as more efficient formation of the immunological synapse, thereby controlling the growth and metastasis of mouse melanoma (284)