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After the reaction was quenched by the addition of 25 μL of 2 N HCl, the reaction mixture was diluted with 100 μL of methanol, and 10 μL of each solution was analyzed through HPLC–MS
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Additionally, defactinib also decreased the viability of normal human hepatic LX-2 cells with a low micromolar IC 50 of 2.19 μM (Fig
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