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After the reaction was quenched by the addition of 25 ΞΌL of 2 N HCl, the reaction mixture was diluted with 100 ΞΌL of methanol, and 10 ΞΌL of each solution was analyzed through HPLCβMS
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Additionally, defactinib also decreased the viability of normal human hepatic LX-2 cells with a low micromolar IC 50 of 2.19 ΞΌM (Fig
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