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discovered that BBR pretreatment significantly inhibited the OGD-induced elevation of p-Bcl-2 levels in organotypic hippocampal brain slice cultures (OHSCs), and this effect was present either before or during simultaneous administration of OGD, indicating that it attenuates neuronal apoptosis by blocking Bcl-2 phosphorylation ( in vitro study demonstrated that BBR could effectively lower intracellular ROS levels in PC12 cells, thereby inhibiting the mitochondrial apoptotic pathway, and thus exerting a neuroprotective effect against OGD-induced PC 12 cell injury (Zhou et al., 2008)