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In this work, five genes ( ldhD, dpoIII, dnaG, gyrA , and gyrB ) were evaluated as internal controls for RT-qPCR, using the primers described in Table 1 (Desroche et al., −ΔΔCT method, and the amount of target RNA was adjusted to the geometric mean of the five internal controls as previously described (Livak and Schmittgen, Table 1 Gene descriptions and the corresponding primer sequences used for validation of RNA-seq results by RT-qPCR

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E., Hanson, D