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To investigate whether one or both putative binding sites were functional, we cloned fragments of 98 and 96 bp of the human IGF-1 3′ UTR mRNA (NM_ 000618) containing the two putative miRNA-binding sites into the psiCHEK-2 vector (Supplementary Fig
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doi: 10.1016/j.fitote.2023.105730 222 ZhouL.SongW.LiuT.YanT.HeZ.HeW.et al
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