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Emerging therapeutic strategies—including small-molecule inhibitors (eg, Dickkopf-1), monoclonal antibodies, and natural compounds like curcumin and Salvia miltiorrhiza extracts—show preclinical promise in modulating Wnt/β-catenin activity
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Briefly, the cells are cross-linked with 1% formaldehyde, lysed and ultrasound treated to produce chromatin fragments of 200−1000 bp, which are immunoprecipitated with IgG or SRF antibodies
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