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To achieve a significant improvement in titer, the in-batch reaction mode was utilized, synthesizing HBA from 5-ALA initially, and then adding the AdoCbl synthesizing enzymes to produce AdoCbl
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Density determination of bacteria and bacterial protoplasts NycodenzⓇ gradients (1.10-1.46 g/ml), or sequential centrifugation in NycodenzⓇ solutions of increasing density, are used to determine the density of bacterial spore protoplasts (e.g
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